CCL25 ELISA Kit

Catalog No. ABIN1889341

Human CCL25 ELISA Kit Colorimetric assay for quantification of Human CCL25.

Quick Overview for CCL25 ELISA Kit (ABIN1889341)

Target: CCL25 (Chemokine (C-C Motif) Ligand 25 (CCL25))

Binding Specificity: AA 24-150

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5-4000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Product Details CCL25 ELISA Kit

Minimum Detection Limit: 62.5 pg/mL

Purpose: For quantitative detection of human CCL25 in cell culture supernates, serum and plasma(heparin, EDTA).

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: Natural and recombinant human CCL25

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <10pg/mL

Components:

• 96-well plate precoated with anti- human CCL25 antibod - 1
• Lyophilized recombinant human CCL25 standar - 10ng/tubex2
• Biotinylated anti- human CCL25 antibod - 130μl(dilution 1:100)
• Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
• Sample diluent buffe - 30ml
• Antibody diluent buffe - 12ml
• ABC diluent buffe - 12ml
• TMB color developing agen - 10ml
• TMB stop solutio - 10ml

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: E.coli
Immunogen sequence: Q24-L150

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plate: Pre-coated

Protocol: human CCL25 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL25 has been precoated onto 96-well plates. Standards(E.coli, Q24-L150) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL25 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CCL25 amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human CCL25 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CCL25 standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 423, Standard deviation: 15.23, CV(%): 3.6
• Sample 2: n=16, Mean(pg/ml): 1094, Standard deviation: 49.23, CV(%): 4.5
• Sample 3: n=16, Mean(pg/ml): 2516, Standard deviation: 158.51, CV(%): 6.3,
• Sample 1: n=24, Mean(pg/ml): 461, Standard deviation: 28.12, CV(%): 6.1
• Sample 2: n=24, Mean(pg/ml): 1273, Standard deviation: 85.29, CV(%): 6.7
• Sample 3: n=24, Mean(pg/ml): 2338, Standard deviation: 168.34, CV(%): 7.2

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

Target Details for CCL25

Target: CCL25 (Chemokine (C-C Motif) Ligand 25 (CCL25))

Alternative Name: CCL25

Background: Chemokine(C-C motif) ligand 25(CCL25) is a small cytokine belonging to the CC chemokine family that is also known as TECK(Thymus-Expressed Chemokine). The mouse Teck gene was mapped to chromosome 8 and human Teck gene was to 19p13.2. CCL25 is believed to play a role in the development of T-cells. It is chemotactic for thymocytes, macrophages, and dendritic cells. CCL25 elicits its effects by binding to the chemokine receptor CCR9

Gene ID: 6370

UniProt: O15444