ACE2 ELISA Kit

Catalog No. ABIN1889360

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse ACE2. There is 1 publication available.

Quick Overview for ACE2 ELISA Kit (ABIN1889360)

Target: ACE2 (Angiotensin I Converting Enzyme 2 (ACE2))

Binding Specificity: AA 18-740

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5-4000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Product Details ACE2 ELISA Kit

Minimum Detection Limit: 62.5 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse ACE2

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: Expression system for standard: CHO
Immunogen sequence: Q18-T740

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <10pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: CHO
Immunogen sequence: Q18-T740

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Tissue Specificity: Expressed in heart, kidney and forebrain (at protein level). Ubiquitously expressed, with highest levels in ileum, kidney and lung. In lung, expressed on vascular endothelial and airway epithelial cells. .

Plate: Pre-coated

Protocol: mouse ACE2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for ACE2 has been precoated onto 96-well plates. Standards(CHO, Q18-T740) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ACE2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse ACE2 amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse ACE2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse ACE2 standard solution and each sample is measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 417, Standard deviation: 20.85, CV(%): 5
• Sample 2: n=16, Mean(pg/ml): 1235, Standard deviation: 65.46, CV(%): 5.3
• Sample 3: n=16, Mean(pg/ml): 2279, Standard deviation: 127.62, CV(%): 5.6,
• Sample 1: n=24, Mean(pg/ml): 503, Standard deviation: 37.73, CV(%): 7.5
• Sample 2: n=24, Mean(pg/ml): 1327, Standard deviation: 102.18, CV(%): 7.7
• Sample 3: n=24, Mean(pg/ml): 2305, Standard deviation: 189.01, CV(%): 8.2

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

References for ACE2 ELISA Kit (ABIN1889360)

Chiang, Liang, Liu, Pan, Chen, Yang, Lee, Tsai, Lee, Chen: "Aliskiren Improves Ischemia- and Oxygen Glucose Deprivation-Induced Cardiac Injury through Activation of Autophagy and AMP-Activated Protein Kinase." in: Frontiers in pharmacology, Vol. 8, pp. 819, (2017) (PubMed).

Target Details for ACE2

Target: ACE2 (Angiotensin I Converting Enzyme 2 (ACE2))

Alternative Name: ACE2

Background:

Protein Function: Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin- 13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. May have a protective role in acute lung injury. .

Background: AngiotensinI-converting enzyme 2(ACE 2) is a protein belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. By sequence similarity to a sequence in GenBank, this gene is mapped to Xp22.2. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63.

Synonyms: Angiotensin-converting enzyme 2,3.4.17.23,ACE-related carboxypeptidase,Processed angiotensin-converting enzyme 2,Ace2,

Full Gene Name: Angiotensin-converting enzyme 2

Cellular Localisation: Processed angiotensin-converting enzyme 2: Secreted.

Gene ID: 70008

UniProt: Q8R0I0

Pathways: ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour