APCS ELISA Kit

Catalog No. ABIN1889378

This Colorimetric ELISA kit is designed for the quantitative measurement of Rat APCS.

Quick Overview for APCS ELISA Kit (ABIN1889378)

Target: APCS (Amyloid P Component, Serum (APCS))

Binding Specificity: AA 21-228

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56-100 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Product Details APCS ELISA Kit

Minimum Detection Limit: 1.56 ng/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat SAP/PTX2

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: Expression system for standard: NSO
Immunogen sequence: Q21-S228

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <50pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: Q21-S228

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Sequence similarities: Belongs to the pentaxin family.

Plate: Pre-coated

Protocol: rat SAP ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for SAP has been precoated onto 96-well plates. Standards(NSO, Q21-S228) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SAP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat SAP amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 100 ng/mL, 50 ng/mL, 25 ng/mL, 1.25 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL rat SAP standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each rat SAP standard solution and each sample is measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(ng/ml): 5.68, Standard deviation: 0.33, CV(%): 5.8
• Sample 2: n=16, Mean(ng/ml): 21, Standard deviation: 0.882, CV(%): 4.2
• Sample 3: n=16, Mean(ng/ml): 42, Standard deviation: 2.1, CV(%): 5,
• Sample 1: n=24, Mean(ng/ml): 5.2, Standard deviation: 0.39, CV(%): 7.5
• Sample 2: n=24, Mean(ng/ml): 24, Standard deviation: 1.296, CV(%): 5.4
• Sample 3: n=24, Mean(ng/ml): 51, Standard deviation: 3.06, CV(%): 6

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

Target Details for APCS

Target: APCS (Amyloid P Component, Serum (APCS))

Alternative Name: APCS

Background:

Background: Amyloid P component, serum(SAP), also known as PTX2 or APCS, is the identical serum form of amyloid P component(AP), a 25 kDa pentameric protein first identified as the pentagonal constituent of in vivo pathological deposits called "amyloid". It belongs to the pentraxins family, characterised by calcium dependent ligand binding and distinctive flattened beta-jellyroll structure similar to that of the legume lectins. This gene is mapped to 1q23.2. The binding of the encoded protein to proteins in the pathological amyloid cross-beta fold suggests its possible role as a chaperone. This protein is also thought to control the degradation of chromatin. It has been demonstrated that this protein binds to apoptotic cells at an early stage, which raises the possibility that it is involved in dealing with apoptotic cells in vivo.

Synonyms: Serum amyloid P-component,SAP,Apcs,Ptx2, Sap,

Full Gene Name: Serum amyloid P-component

Cellular Localisation: Secreted.

Gene ID: 29339

UniProt: P23680