CD56 ELISA Kit

Catalog No. ABIN1889387

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse CD56.

Quick Overview for CD56 ELISA Kit (ABIN1889387)

Target: CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))

Binding Specificity: AA 20-711

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 625-40.000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Product Details CD56 ELISA Kit

Minimum Detection Limit: 625 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CD56/NCAM1

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: Expression system for standard: NSO
Immunogen sequence: L20-T711

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <20pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: L20-T711

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Sequence similarities: Contains 2 fibronectin type-III domains.

Plate: Pre-coated

Protocol: mouse CD56 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD56 has been precoated onto 96-well plates. Standards(NSO, L20-T711) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD56 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD56 amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 40,000pg/mL, 20,000pg/mL, 10,000pg/mL, 5,000pg/mL, 2,500pg/mL, 1,250pg/mL, 625pg/mL mouse CD56 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse CD56 standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(ng/ml): 6.7, Standard deviation: 0.281, CV(%): 4.2
• Sample 2: n=16, Mean(ng/ml): 17.2, Standard deviation: 0.946, CV(%): 5.5
• Sample 3: n=16, Mean(ng/ml): 28.6, Standard deviation: 1.83, CV(%): 6.4,
• Sample 1: n=24, Mean(ng/ml): 8.3, Standard deviation: 3.984, CV(%): 4.8
• Sample 2: n=24, Mean(ng/ml): 19.5, Standard deviation: 1.326, CV(%): 6.8
• Sample 3: n=24, Mean(ng/ml): 32.5, Standard deviation: 2.34, CV(%): 7.2

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

Target Details for CD56

Target: CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))

Alternative Name: NCAM1

Background:

Protein Function: This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.

Background: Neural Cell Adhesion Molecule, NCAM, also known as the cluster of differentiation CD56, is a hemophilic binding glycoprotein. It is a glycoprotein of Immunoglobulin(Ig) super family. At least 27 alternatively spliced NCAM mRNAs are produced, giving a wide diversity of NCAM isoforms NCAM gene is located at 11q22-q23.This glycoprotein is mainly expressed on the surface of neurons, glia, skeletal muscle and natural killer cells. NCAM has been implicated as having a role in cellâ€"cell adhesion, neurite outgrowth, synaptic plasticity, and learning and memory.

Synonyms: Neural cell adhesion molecule 1,N-CAM-1,NCAM-1,CD56,Ncam1,Ncam,

Full Gene Name: Neural cell adhesion molecule 1

Cellular Localisation: Isoform 1: Cell membrane, Single-pass type I membrane protein.

Gene ID: 17967

UniProt: P13595