GAL4 ELISA Kit

Catalog No. ABIN1889417

This Colorimetric ELISA kit is designed for the quantitative measurement of Human GAL4.

Quick Overview for GAL4 ELISA Kit (ABIN1889417)

Target: GAL4 (LGALS4) (Galectin 4 (LGALS4))

Binding Specificity: AA 2-323

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156-10.000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Product Details GAL4 ELISA Kit

Minimum Detection Limit: 156 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Galectin-4

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: Expression system for standard: E.coli
Immunogen sequence: A2-I323

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <10pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: E.coli
Immunogen sequence: A2-I323

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Sequence similarities: Contains 2 galectin domains.

Plate: Pre-coated

Protocol: human Galectin-4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Galectin-4 has been precoated onto 96-well plates. Standards(E.coli, A2-I323) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Galectin-4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Galectin-4 amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Galectin-4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Galectin-4 standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(ng/ml): 0.48, Standard deviation: 0.02, CV(%): 4.2
• Sample 2: n=16, Mean(ng/ml): 3.64, Standard deviation: 0.14, CV(%): 3.8
• Sample 3: n=16, Mean(ng/ml): 6.25, Standard deviation: 0.35, CV(%): 5.6,
• Sample 1: n=24, Mean(ng/ml): 0.51, Standard deviation: 0.03, CV(%): 5.9
• Sample 2: n=24, Mean(ng/ml): 4.02, Standard deviation: 0.19, CV(%): 4.7
• Sample 3: n=24, Mean(ng/ml): 6.93, Standard deviation: 0.43, CV(%): 6.2

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

Target Details for GAL4

Target: GAL4 (LGALS4) (Galectin 4 (LGALS4))

Alternative Name: LGALS4

Background:

Protein Function: Galectin that binds lactose and a related range of sugars. May be involved in the assembly of adherens junctions.

Background: Galectin-4, also known as LGALS4 or GAL4, is a protein that in humans is encoded by the LGALS4 gene. It is mapped to 19q13.2. Galectins are a family of animal lectins defined by an affinity for beta-galactoside-containing saccharides and by shared sequence elements. Galectin-4 is expressed as a 38-kD protein in the human colon adenocarcinoma T84 cell line. Galectin-4 is an S-type lectin that is strongly underexpressed in colorectal cancer, and this gene plays a role in cell adhesion. It has been found that decreased Galectin-4 expression occurs early in colorectal carcinogenesis.

Synonyms: Galectin-4,Gal-4,Antigen NY-CO-27,L-36 lactose-binding protein,L36LBP,Lactose-binding lectin 4,LGALS4,

Full Gene Name: Galectin-4

Gene ID: 3960

UniProt: P56470