LGALS7 ELISA Kit

Catalog No. ABIN1889421

This Mouse LGALS7 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Mouse LGALS7.

Quick Overview for LGALS7 ELISA Kit (ABIN1889421)

Target: LGALS7 (Galectin 7 (LGALS7))

Binding Specificity: AA 2-136

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 93.8-6000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Product Details LGALS7 ELISA Kit

Minimum Detection Limit: 93.8 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Galectin-7

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: Expression system for standard: E.coli
Immunogen sequence: S2-F136

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <10pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: E.coli
Immunogen sequence: S2-F136

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plate: Pre-coated

Protocol: mouse Galectin-7 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Galectin-7 has been precoated onto 96-well plates. Standards(E.coli, S2-F136) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Galectin-7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Galectin-7 amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 6000pg/mL, 3000pg/mL, 1500pg/mL, 750pg/mL, 375pg/mL, 187.5pg/mL, 93.8pg/mL mouse Galectin-7 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse Galectin-7 standard solution and each sample is measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 356, Standard deviation: 16.4, CV(%): 4.6
• Sample 2: n=16, Mean(pg/ml): 2745, Standard deviation: 156.5, CV(%): 5.7
• Sample 3: n=16, Mean(pg/ml): 5032, Standard deviation: 191.2, CV(%): 3.8,
• Sample 1: n=24, Mean(pg/ml): 468, Standard deviation: 33.7, CV(%): 7.2
• Sample 2: n=24, Mean(pg/ml): 2012, Standard deviation: 1710.2, CV(%): 8.5
• Sample 3: n=24, Mean(pg/ml): 4584, Standard deviation: 289, CV(%): 6.3

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

Target Details for LGALS7

Target: LGALS7 (Galectin 7 (LGALS7))

Alternative Name: LGALS7

Background:

Protein Function: Could be involved in cell-cell and/or cell-matrix interactions necessary for normal growth control. Pro-apoptotic protein that functions intracellularly upstream of JNK activation and cytochrome c release (By similarity). .

Background: Galectin-7, also known as GAL7, is a protein that in humans is encoded by the LGALS7 gene. This gene is mapped to 19q13.2. Galectins belong to a family of related beta-galactoside-binding lectins, also referred to as S-type or S-Lac lectins. Members of this family have been implicated in a variety of functions, including growth regulation, cell adhesion, migration, neoplastic transformation, and immune responses. Differential and in situ hybridizations indicate that this lectin is specifically expressed in keratinocytes. It has been found that expression of Galectin-7 was markedly upregulated in corneal epithelium after injury and that exogenous galectin-7 stimulated reepithelialization of corneal wounds.

Synonyms: Galectin-7,Gal-7,Lgals7,

Full Gene Name: Galectin-7

Cellular Localisation: Cytoplasm . Nucleus . Secreted . May be secreted by a non-classical secretory pathway..

Gene ID: 16858

UniProt: O54974