Cyclic GMP ELISA Kit

Catalog No. ABIN7540317

Chemical Cyclic GMP ELISA Kit Colorimetric assay for quantification of Chemical Cyclic GMP.

Quick Overview for Cyclic GMP ELISA Kit (ABIN7540317)

Target: Cyclic GMP (cGMP)

Reactivity: Chemical

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 0.3-66.7 pM/mL

Application: ELISA

Sample Type: Serum, Plasma, Saliva, Cell Culture Supernatant, Urine

Product Details Cyclic GMP ELISA Kit

Minimum Detection Limit: 0.3 pM/mL

Purpose: Quantitative detection of cGMP in samples such as serum, plasma, saliva, cell culture supernatant, and urine.

Analytical Method: Quantitative

Specificity: Easy - use: All reagents and buffers for cGMP test are provided.

Cross-Reactivity (Details): No significant cross-reactivity of similar compounds was found.

Sensitivity: 0.11 pmol/mL

Characteristics: cGMP ELISA Detection Kit is a competition enzyme-linked immunoassay which can be used for quantitative detection of cGMP (Guanosine 3',5'-cyclic monophosphate) in samples such as serum, plasma, saliva, cell culture supernatant, and urine. cGMP is an important secondary messenger in signal transduction pathways. It is a common regulator of ion channel conductance, glycogenolysis, and cellular apoptosis. cGMP is a cyclic nucleotide derived from guanosine triphosphate (GTP). The cellular production of cGMP is mediated by Guanylate cyclase (GC). It activates the cGMP-dependent protein kinases which in turn phosphorylate several downstream protein targets. cGMP has an effect on the regulation of cAMP levels by activating or inhibiting specific phosphodiesterases (PDEs). The anti-IgG Capture Plate is pre-coated with fixed amount of Goat anti-rabbit IgG to capture Rabbit Anti-cGMP Polyclonal Antibody. When free cGMP or specimen and HRP-cGMP conjugate are added to the well, they compete in the solution to interact with the cGMP antibody captured on the plate. Other unbound molecules are removed by a wash step. The cGMP-HRP reacts with TMB substrate to develop a blue product in the solution. The reaction is stopped by adding stop solution and the color turns yellow which can be read at 450 nm by a Microtiter plate reader. Using the standard curve, the amount of cGMP present in the unknown samples can be calculated by transforming its absorbance value.

Components: Anti-IgG Capture Plate: 1 plate (8 wells x 12 strips)
Anti-cGMP pAb: 12 ml
HRP-cGMP: 6 ml
cGMP Standards (0, 0.3, 0.8, 2.5, 7.4, 22.2, 66.7 pmol/ml): 1.5 ml
cGMP Standard Stock (10 nmol/ml): 500 μl
Assay Buffer A: 60 ml
Assay Buffer B: 1 ml
20 × Wash Solution: 40 ml
TMB Substrate: 12 ml
Stop Solution: 6 ml
Plate Sealer: 2 pieces
User Manual: 1 copy

Application Details

Assay Time: 2.5 h

Plate: Pre-coated

Protocol: The anti-IgG Capture Plate is pre-coated with fixed amount of Goat anti-rabbit IgG to capture Rabbit Anti-cGMP Polyclonal Antibody. When free cGMP or specimen and HRP-cGMP conjugate are added to the well, they compete in the solution to interact with the cGMP antibody captured on the plate. Other unbound molecules are removed by a wash step. The cGMP-HRP reacts with TMB substrate to develop a blue product in the solution. The reaction is stopped by adding stop solution and the color turns yellow which can be read at 450 nm by a Microtiter plate reader. Using the standard curve, the amount of cGMP present in the unknown samples can be calculated by transforming its absorbance value.

Restrictions: For Research Use only

Handling

Handling Advice: Do not freeze the kit.

Storage: 4 °C

Storage Comment: The unopened kit is stable for at least 12 months if stored at 2-8 °C, and the opened kit is stable for up to 2 weeksat 2-8 °C.

Expiry Date: 12 months

Target Details for Cyclic GMP

Target: Cyclic GMP (cGMP)

Alternative Name: cGMP

Target Type: Chemical

Background: CGMP is an important secondary messenger in signal transduction pathways. It is a common regulator of ion channel conductance, glycogenolysis, and cellular apoptosis. cGMP is a cyclic nucleotide derived from guanosine triphosphate (GTP). The cellular production of cGMP is mediated by Guanylate cyclase (GC). It activates the cGMP-dependent protein kinases which in turn phosphorylate several downstream protein targets. cGMP has an effect on the regulation of cAMP levels by activating or inhibiting specific phosphodiesterases (PDEs).