PRSS1 ELISA Kit

Catalog No. ABIN2042276

This Colorimetric ELISA kit is designed for the quantitative measurement of Human PRSS1.

Quick Overview for PRSS1 ELISA Kit (ABIN2042276)

Target: PRSS1 (Protease, serine, 1 (Trypsin 1) (PRSS1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.78-50 ng/mL

Application: ELISA

Product Details PRSS1 ELISA Kit

Minimum Detection Limit: 0.78 ng/mL

Sensitivity: < 0.36 ng/mL

Application Details

Plate: Pre-coated

Protocol: Protease, Serine 1 (PRSS1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protease, Serine 1 (PRSS1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protease, Serine 1 (PRSS1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protease, Serine 1 (PRSS1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Protease, Serine 1 (PRSS1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for PRSS1

Target: PRSS1 (Protease, serine, 1 (Trypsin 1) (PRSS1))

Alternative Name: Protease, Serine 1 (PRSS1)