ACVA ELISA Kit

Catalog No. ABIN2042339

This Colorimetric ELISA kit is designed for the quantitative measurement of Rat ACVA.

Quick Overview for ACVA ELISA Kit (ABIN2042339)

Target: ACVA (Activin A (ACVA))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5-4000 pg/mL

Application: ELISA

Product Details ACVA ELISA Kit

Minimum Detection Limit: 62.5 pg/mL

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 12 pg/mL

Components: 96-well plate pre-coated with anti-Activin A, Rat
Lyophilized recombinant Activin A, Rat standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Application Details

Plate: Pre-coated

Protocol: Sandwich ELISA quantitative detection kit for Rat Activin A in cell culture supernates, serum, plasma (heparin, EDTA) and saliva.Activin A is a homodimer of 14kD beta-A. Activin A, a cytokine member of the transforming growth factor-beta superfamily, is expressed locally by the mesenchymal component of the hemopoietic microenvironment. Its expression is regulated on the mRNA level by different cytokines, and the biological activity of the protein is tightly controlled by several inhibitory molecules.1 Inhibins and activins are members of the transforming growth factor beta superfamily and are known to modulate the growth and differentiation of several cell types.2 Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. The standard product used in this kit is recombinant Activin A, which is composed of two single chains of 116 amino acids with the molecular mass of 26kD.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for ACVA

Target: ACVA (Activin A (ACVA))

Alternative Name: Activin A

Gene ID: 29200

UniProt: P18331

Pathways: Hormone Transport, Peptide Hormone Metabolism