Cathepsin B ELISA Kit

Catalog No. ABIN2042370

This Human Cathepsin B ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human Cathepsin B.

Quick Overview for Cathepsin B ELISA Kit (ABIN2042370)

Target: Cathepsin B (CTSB)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 6-10000 pg/mL

Application: ELISA

Product Details Cathepsin B ELISA Kit

Minimum Detection Limit: 6 pg/mL

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 5 pg/mL

Components: 96-well plate pre-coated with anti-Cathepsin B, Human
Lyophilized recombinant Cathepsin B, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Application Details

Plate: Pre-coated

Protocol: Sandwich ELISA quantitative detection kit for Human Cathepsin B in cell culture supernates, serum and plasma (heparin, EDTA).Cathepsin B is an enzymatic protein belonging to the peptidase or protease families. In humans, it is coded by the CTSB gene.1, 2 And this gene is mapped to chromosome 8p22.3 The protein encoded by this gene is a lysosomal cysteine proteinase composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. It is a member of the peptidase C1 family. Cathepsin B was once suspected as a candidate protease participating in the conversion of -amyloid precursor protein into the amyloid plaques found in Alzheimer's disease patients. However, this function is now known to be mediated by BACE1 protease. It is now thought that cathepsin B can degrade -amyloid precursor protein into harmless fragments. Thus, it is conceivable cathepsin B may play a pivotal role in the natural defense against Alzheimer's disease.4 Overexpression of cathepsin B has been associated with esophageal adenocarcinoma and other tumors. At least five transcript variants encoding the same protein have been found for this gene. The standard product used in this kit is recombinant human Cathepsin B with the molecular mass of 37kD.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for Cathepsin B

Target: Cathepsin B (CTSB)

Alternative Name: Cathepsin B

Gene ID: 1508

UniProt: P07858

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades