CCL21 ELISA Kit

Catalog No. ABIN2042380

Human CCL21 ELISA Kit Colorimetric assay for quantification of Human CCL21.

Quick Overview for CCL21 ELISA Kit (ABIN2042380)

Target: CCL21 (Chemokine (C-C Motif) Ligand 21 (CCL21))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5-4000 pg/mL

Application: ELISA

Product Details CCL21 ELISA Kit

Minimum Detection Limit: 62.5 pg/mL

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 12 pg/mL

Components: 96-well plate pre-coated with anti-CCL21/6Ckine, Human
Lyophilized recombinant CCL21/6Ckine, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Application Details

Plate: Pre-coated

Protocol: Sandwich ELISA quantitative detection kit for Human CCL21/6Ckine in cell culture supernates. serum and plasma (heparin, EDTA).Chemokine (C-C motif) ligand 21 (CCL21) is a small cytokine belonging to the CC chemokine family. This chemokine is also known as 6Ckine (because it has six conserved cysteine residues instead of the four cysteines typical to chemokines), exodus-2, and secondary lymphoid-tissue chemokine (SLC).1 By somatic cell hybrid and radiation hybrid analyses, mapped the SCYA21 gene to 9p13. 2 Chemokines are a family of proteins that direct leukocyte migration and activation to inflammatory stimuli. CXC chemokine ligand 13 (CXCL13), CC chemokine ligand 21 (CCL21), and CCL19 are constitutively expressed in secondary lymphoid organs, where they control the placement of lymphocytes and dendritic cells. It was demonstrate that the local expression of homeostatic chemokines in nonlymphoid organs, such as the lung, plays an important role in protective immune responses.3

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for CCL21

Target: CCL21 (Chemokine (C-C Motif) Ligand 21 (CCL21))

Alternative Name: CCL21/6Ckine

Gene ID: 6366

UniProt: O00585

Pathways: Regulation of Actin Filament Polymerization