CCL4 ELISA Kit
Quick Overview for CCL4 ELISA Kit (ABIN2042385)
Target
See all CCL4 ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
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Minimum Detection Limit
- 15.6 pg/mL
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Cross-Reactivity (Details)
- There is no detectable cross-reactivity with other relevant proteins.
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Sensitivity
- < 10 pg/mL
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Components
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96-well plate pre-coated with anti-CCL4/MIP-1 beta, Rat
Lyophilized recombinant CCL4/MIP-1 beta, Rat standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
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Plate
- Pre-coated
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Protocol
- Sandwich ELISA quantitative detection kit for Rat CCL4/MIP-1beta in cell culture supernates, serum and plasma (heparin, EDTA, citrate).Chemokine (C-C motif) ligand 4, also known as CCL4, is a protein which in mouse is encoded by the CCL4 gene. It is a CC chemokine with specificity for CCR5 receptors. It is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. CCL4 is a major HIV-suppressive factor produced by CD8+ T cells. Performing-low memory CD8+ T cells that normally synthesize MIP-1-beta. By analysis of somatic cell hybrids and by in situ hybridization, the CCL4 gene was assigned to 17q21-q23. Based on functional analysis of MIP-1 , one or more additional chemokine receptors that transduce MIP-1 signals must also exist. MIP-1 and MIP-1 have been shown to lack affinity for the erythrocyte surface chemokine receptor/Duffy antigen that binds many as well as chemokines (11, 14).
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Restrictions
- For Research Use only
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Storage
- 4 °C/-20 °C
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- CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))
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Alternative Name
- CCL4/MIP-1 beta
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Gene ID
- 116637
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UniProt
- P50230
Target See all CCL4 ELISA Kits
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