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CCL4 ELISA Kit

This Rat CCL4 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Rat CCL4.
Catalog No. ABIN2042385

Quick Overview for CCL4 ELISA Kit (ABIN2042385)

Target

See all CCL4 ELISA Kits
CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))

Reactivity

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  • 1
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Rat

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

15.6-1000 pg/mL

Application

ELISA
  • Minimum Detection Limit

    15.6 pg/mL

    Cross-Reactivity (Details)

    There is no detectable cross-reactivity with other relevant proteins.

    Sensitivity

    < 10 pg/mL

    Components

    96-well plate pre-coated with anti-CCL4/MIP-1 beta, Rat
    Lyophilized recombinant CCL4/MIP-1 beta, Rat standard, 10 ng/tube, 2Ea
    Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
    Sample diluent buffer, 30 mL
    Antibody diluent buffer, 10 mL
    ABC diluent buffer, 10 mL
    TMB color developing agent, 10 mL
    TMB stop solution, 10 mL
  • Plate

    Pre-coated

    Protocol

    Sandwich ELISA quantitative detection kit for Rat CCL4/MIP-1beta in cell culture supernates, serum and plasma (heparin, EDTA, citrate).Chemokine (C-C motif) ligand 4, also known as CCL4, is a protein which in mouse is encoded by the CCL4 gene. It is a CC chemokine with specificity for CCR5 receptors. It is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. CCL4 is a major HIV-suppressive factor produced by CD8+ T cells. Performing-low memory CD8+ T cells that normally synthesize MIP-1-beta. By analysis of somatic cell hybrids and by in situ hybridization, the CCL4 gene was assigned to 17q21-q23. Based on functional analysis of MIP-1 , one or more additional chemokine receptors that transduce MIP-1 signals must also exist. MIP-1 and MIP-1 have been shown to lack affinity for the erythrocyte surface chemokine receptor/Duffy antigen that binds many as well as chemokines (11, 14).

    Restrictions

    For Research Use only
  • Storage

    4 °C/-20 °C
  • Target See all CCL4 ELISA Kits

    CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))

    Alternative Name

    CCL4/MIP-1 beta

    Gene ID

    116637

    UniProt

    P50230
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