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CD40 ELISA Kit

This Human CD40 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human CD40.
Catalog No. ABIN2042400

Quick Overview for CD40 ELISA Kit (ABIN2042400)

Target

See all CD40 ELISA Kits
CD40

Reactivity

  • 10
  • 10
  • 4
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Human

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

15.6-1000 pg/mL

Application

ELISA
  • Minimum Detection Limit

    15.6 pg/mL

    Cross-Reactivity (Details)

    There is no detectable cross-reactivity with other relevant proteins.

    Sensitivity

    < 1 pg/mL

    Components

    96-well plate pre-coated with anti-CD40/TNFRSF5, Human
    Lyophilized recombinant CD40/TNFRSF5, Human standard, 10 ng/tube, 2Ea
    Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
    Sample diluent buffer, 30 mL
    Antibody diluent buffer, 10 mL
    ABC diluent buffer, 10 mL
    TMB color developing agent, 10 mL
    TMB stop solution, 10 mL
  • Plate

    Pre-coated

    Protocol

    Sandwich ELISA quantitative detection kit for Human CD40 in cell culture supernates, serum, plasma ( eparin, EDTA), saliva, urine and human milk.CD40 is a cell surface receptor that is expressed on the surface of all mature B cells, most mature B-cell malignancies, and some early B-cell acute lymphocytic leukemias, but is not expressed on plasma cells.1The CD40 gene is localized to the long arm of chromosome 20, bands q12-q13.2.2The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation.3

    Restrictions

    For Research Use only
  • Storage

    4 °C/-20 °C
  • Target See all CD40 ELISA Kits

    CD40

    Alternative Name

    CD40/TNFRSF5

    Gene ID

    958

    UniProt

    P25942

    Pathways

    NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
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