PF4 ELISA Kit
Quick Overview for PF4 ELISA Kit (ABIN2042428)
Target
See all PF4 ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
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Minimum Detection Limit
- 15.6 pg/mL
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Cross-Reactivity (Details)
- There is no detectable cross-reactivity with other relevant proteins.
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Sensitivity
- < 1 pg/mL
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Components
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96-well plate pre-coated with anti-CXCL4/PF4, Mouse
Lyophilized recombinant CXCL4/PF4, Mouse standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
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Platelet Factor 4 (PF4) ELISA Kit
PF4 Reactivity: Human Colorimetric Sandwich ELISA 1.56 pg/mL - 100 pg/mL Cell Culture Supernatant, Platelet-Poor Plasma, Serum
Platelet Factor 4 (PF4) ELISA KitPF4 Reactivity: Mouse Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Platelet-Poor Plasma, Serum, Tissue Homogenate
Platelet Factor 4 (PF4) ELISA KitPF4 Reactivity: Rat Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Platelet-Poor Plasma, Serum
Platelet Factor 4 (PF4) ELISA KitPF4 Reactivity: Mouse Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Platelet Factor 4 (PF4) ELISA KitPF4 Reactivity: Human Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Platelet Factor 4 (PF4) ELISA KitPF4 Reactivity: Rat Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Platelet Factor 4 (PF4) ELISA KitPF4 Reactivity: Rabbit Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Platelet-Poor Plasma, Serum
Platelet Factor 4 (PF4) ELISA KitPF4 Reactivity: Cow Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Cell Culture Supernatant, Cell Lysate, Platelet-Poor Plasma, Serum, Tissue Homogenate
Platelet Factor 4 (PF4) ELISA KitPF4 Reactivity: Pig Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma
Platelet Factor 4 (PF4) ELISA KitPF4 Reactivity: Mouse AA 30-105 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
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Plate
- Pre-coated
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Protocol
- Sandwich ELISA quantitative detection kit for Mouse CXCL4/PF4 in cell culture supernates, serum and plasma (heparin, EDTA).Platelet factor 4 (PF4) is a small cytokine belonging to the CXC chemokine family that is also known as chemokine (C-X-C motif) ligand 4 (CXCL4). The PF4 gene was localized on map to 4q12-q13. Chemokines play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis. Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair.
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Restrictions
- For Research Use only
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Storage
- 4 °C/-20 °C
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- PF4 (Platelet Factor 4 (PF4))
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Alternative Name
- CXCL4/PF4
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Gene ID
- 56744
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UniProt
- Q9Z126
Target See all PF4 ELISA Kits
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