CST3 ELISA Kit

Catalog No. ABIN2042433

This Human CST3 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human CST3.

Quick Overview for CST3 ELISA Kit (ABIN2042433)

Target: CST3 (Cystatin C (CST3))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 2-20000 pg/mL

Application: ELISA

Product Details CST3 ELISA Kit

Minimum Detection Limit: 2 pg/mL

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components: 96-well plate pre-coated with anti-Cystatin C, Human
Lyophilized recombinant Cystatin C, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Application Details

Plate: Pre-coated

Protocol: Sandwich ELISA quantitative detection kit for Human Cystatin C in cell culture supernates, serum, plasma ( eparin, EDTA), saliva, urine and human milk.Cystatin C or cystatin 3 (formerly gamma trace, post-gamma-globulin or neuroendocrine basic polypeptide), a protein encoded by the CST3 gene, was originally described as a constituent of normal cerebrospinal fluid (CSF) and of urine from patients with renal failure.1 Cystatin 3 has a low molecular weight (approximately 13.3 kilodaltons), and it is removed from the bloodstream by glomerular filtration in the kidneys. In humans, all cells with a nucleus (cell core containing the DNA) produce cystatin C as a chain of 120 amino acids. It is found in virtually all tissues and bodily fluids. Cystatin C, which belongs to the type II cystatin gene family, is a potent inhibitor of lysosomal proteinases2 (enzymes from a special subunit of the cell that break down proteins) and probably one of the most important extracellular inhibitors of cysteine proteases3 (it prevents the breakdown of proteins outside the cell by a specific type of protein degrading enzymes). Moreover, cystatin C is involved in network reorganization in the epileptic dentate gyrus.4

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for CST3

Target: CST3 (Cystatin C (CST3))

Alternative Name: Cystatin C

Gene ID: 1471

UniProt: P01034