CST3 ELISA Kit
Quick Overview for CST3 ELISA Kit (ABIN2042434)
Target
See all CST3 ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
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Minimum Detection Limit
- 2 pg/mL
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Cross-Reactivity (Details)
- There is no detectable cross-reactivity with other relevant proteins.
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Sensitivity
- < 10 pg/mL
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Components
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96-well plate pre-coated with anti-Cystatin C, Mouse
Lyophilized recombinant Cystatin C, Mouse standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
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Cystatin C (CST3) ELISA Kit
CST3 Reactivity: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Cystatin C (CST3) ELISA KitCST3 Reactivity: Human Colorimetric Sandwich ELISA 7.8-500 ng/mL Urine
Cystatin C (CST3) ELISA KitCST3 Reactivity: Mouse Colorimetric Sandwich ELISA 2.5-160 ng/mL Plasma, Serum, Tissue Homogenate
Cystatin C (CST3) ELISA KitCST3 Reactivity: Mouse Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Cystatin C (CST3) ELISA KitCST3 Reactivity: Rat Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Urine
Cystatin C (CST3) ELISA KitCST3 Reactivity: Pig Colorimetric Sandwich ELISA 125 ng/mL - 8000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Cystatin C (CST3) ELISA KitCST3 Reactivity: Rat Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Cystatin C (CST3) ELISA KitCST3 Reactivity: Monkey Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Cystatin C (CST3) ELISA KitCST3 Reactivity: Cow Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Cystatin C (CST3) ELISA KitCST3 Reactivity: Human AA 1-146 Colorimetric Sandwich ELISA 312-20000 pg/mL Cell Culture Supernatant, Milk, Plasma (EDTA), Plasma (heparin), Saliva, Serum, Urine
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Plate
- Pre-coated
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Protocol
- Sandwich ELISA quantitative detection kit for Mouse Cystatin C in cell culture supernates, serum, plasma (heparin, EDTA) and urine.Cystatin C or cystatin 3 (formerly gamma trace, post-gamma-globulin or neuroendocrine basic polypeptide), a protein encoded by the CST3 gene, was originally described as a constituent of normal cerebrospinal fluid (CSF) and of urine from patients with renal failure. Cystatin 3 has a low molecular weight (approximately 13.3 kilodaltons), and it is removed from the bloodstream by glomerular filtration in the kidneys. In mouses, all cells with a nucleus (cell core containing the DNA) produce cystatin C as a chain of 120 amino acids. It is found in virtually all tissues and bodily fluids. Cystatin C, which belongs to the type II cystatin gene family, is a potent inhibitor of lysosomal proteinases (enzymes from a special subunit of the cell that break down proteins) and probably one of the most important extracellular inhibitors of cysteine proteases (it prevents the breakdown of proteins outside the cell by a specific type of protein degrading enzymes). Moreover, cystatin C is involved in network reorganization in the epileptic dentate gyrus.
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Restrictions
- For Research Use only
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Storage
- 4 °C/-20 °C
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- CST3 (Cystatin C (CST3))
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Alternative Name
- Cystatin C
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Gene ID
- 13010
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UniProt
- P21460
Target See all CST3 ELISA Kits
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