Endothelin ELISA Kit

Catalog No. ABIN2042450

This Colorimetric ELISA kit is designed for the quantitative measurement of Human Endothelin.

Quick Overview for Endothelin ELISA Kit (ABIN2042450)

Target: Endothelin

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 3.9-250 pg/mL

Application: ELISA

Product Details Endothelin ELISA Kit

Minimum Detection Limit: 3.9 pg/mL

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 0.5 pg/mL

Components: 96-well plate pre-coated with anti-Endothelin, Human
Lyophilized recombinant Endothelin, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Application Details

Plate: Pre-coated

Protocol: Sandwich ELISA quantitative detection kit for Human Endothelin in cell culture supernates, serum and plasma (heparin, EDTA).The endothelins is a family of structurally and pharmacologically distinct peptides, which has been identified and sequenced in humans. Three isoforms of human endothelin have been identified endothelin-1, -2, and -3. Endothelin-1 is a potent, 21-amino acid vasoconstrictor peptide produced by vascular endothelial cells. Endothelins are 21-amino acid vasoconstricting peptides produced primarily in the endothelium having a key role in vascular homeostasis. Endothelins are implicated in vascular diseases of several organ systems, including the heart, general circulation and brain. Endothelins are proteins that constrict blood vessels and raise blood pressure. They are normally kept in balance by other mechanisms, but when they are over-expressed, they contribute to high blood pressure (hypertension) and heart disease.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for Endothelin

Target: Endothelin

Gene ID: 1906

UniProt: P05305