FASL ELISA Kit

Catalog No. ABIN2042460

This Human FASL ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human FASL.

Quick Overview for FASL ELISA Kit (ABIN2042460)

Target: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 15.6-1000 pg/mL

Application: ELISA

Product Details FASL ELISA Kit

Minimum Detection Limit: 15.6 pg/mL

Brand: BioAssay™

Cross-Reactivity (Details): No detectable crossreactivity with other relevant proteins

Sensitivity: < 2 pg/mL

Components: A: Microtiter Strip, 1x96 wells
B: FASL Standard, 2x10 ng
C: Anti-human FASL (Biotin), 130 µL
: Avidin-Biotin-Peroxidase Complex (ABC), 1x130 µL (1:100 dilution)
: Sample diluent buffer, 1x30 mL
: Antibody diluent buffer, 1x10 mL
: ABC diluent buffer, 1x10 mL
: TMB color developing agent, 1x10 mL
: TMB stop solution, 2N H2SO4, 1x10 mL

Application Details

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for FASL

Target: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Alternative Name: FASL

Background: Fas ligand (FasL or CD95L) is a type-II transmembrane protein that belongs to the tumor necrosis factor (TNF) family. Its binding with its receptor induces apoptosis. The human FASL gene consists of approximately 8kb and is split into 4 exons. Fas ligand/receptor interactions play an important role in the regulation of the immune system and the progression of cancer. Fas ligand or FasL is a homotrimeric type II transmembrane protein. It signals through trimerization of FasR, which spans the membrane of the "target" cell. This trimerization usually leads to apoptosis, or cell death. Soluble Fas ligand is generated by cleaving membrane-bound FasL at a conserved cleavage site by the external matrix metalloproteinase MMP-7.

Pathways: Apoptosis, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity