FASL ELISA Kit

Catalog No. ABIN1672725

The Human FASL ELISA Kit (ABIN1672725) is a Colorimetric ELISA Kit designed to quantify Human FASL. This ELISA Kit has been cited in 4 publications.

Quick Overview for FASL ELISA Kit (ABIN1672725)

Target: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Binding Specificity: AA 134-281

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6 pg/mL - 1000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)

Product Details FASL ELISA Kit

Minimum Detection Limit: 15.6 pg/mL

Purpose: Sandwich Human FASL / CD95LG / CD95 Ligand ELISA Kit to quantitate Human FASLG in cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Analytical Method: Quantitative

Specificity: Expression system for standard: CHO
Immunogen sequence: P134-L281

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 2 pg/mL

Components:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Assay Precision: Intra-Assay Precision (CV%): 6.1%, 5.2%, 6.6%
Inter-Assay Precision (CV%): 6.2%, 6.6%, 7.9%

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Expiry Date: 12 months

References for FASL ELISA Kit (ABIN1672725)

Xu, Liu, Wu, Gong, Zhou, Qiao: "Proapoptotic effect of metalloproteinase 9 secreted by trophoblasts on endothelial cells." in: The journal of obstetrics and gynaecology research, Vol. 37, Issue 3, pp. 187-94, (2011) (PubMed).

Xiong, Lu, Zhao, Xu, Bao, Lin, Yang: "Therapy with FasL-gene-modified dendritic cells confers a protective microenvironment in murine pregnancy." in: Fertility and sterility, Vol. 93, Issue 8, pp. 2767-9, (2010) (PubMed).

Li, Peng, Li, Liu, Liang, Zhang: "Clinical significance of Fas and FasL protein expression in gastric carcinoma and local lymph node tissues." in: World journal of gastroenterology, Vol. 16, Issue 10, pp. 1274-8, (2010) (PubMed).

Han, Qiu, Zhang, Kong, Wang, Wang, Li, Duan, Wang, Song, Wang: "Transplantation of sertoli-islet cell aggregates formed by microgravity: prolonged survival in diabetic rats." in: Experimental biology and medicine (Maywood, N.J.), Vol. 234, Issue 5, pp. 595-603, (2009) (PubMed).

Target Details for FASL

Target: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Alternative Name: FASLG

Background:

Background: Fas ligand (FasL or CD95L) is a type-II transmembrane protein that belongs to the tumor necrosis factor (TNF) family. Its binding with its receptor induces apoptosis. The human FASL gene consists of approximately 8 kb and is split into 4 exons. Fas ligand/receptor interactions play an important role in the regulation of the immune system and the progression of cancer. Fas ligand or FasL is a homotrimeric type II transmembrane protein. It signals through trimerization of FasR, which spans the membrane of the target cell. This trimerization usually leads to apoptosis, or cell death. Soluble Fas ligand is generated by cleaving membrane-bound FasL at a conserved cleavage site by the external matrix metalloproteinase MMP-7.

Gene Full Name: Fas ligand

Gene ID: 356

UniProt: P48023

Pathways: Apoptosis, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity