Fibronectin ELISA Kit
Quick Overview for Fibronectin ELISA Kit (ABIN2042466)
Target
See all Fibronectin ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
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Minimum Detection Limit
- 156 pg/mL
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Cross-Reactivity (Details)
- No detectable crossreactivity with other relevant proteins
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Sensitivity
- < 10 pg/mL
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Components
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A: Microtiter Strip, 1x96 wells
B: Fibronectin Standard, 2x10 ng
C: Anti-human Fibronectin (Biotin), 1x130 µL
: Avidin-Biotin-Peroxidase Complex (ABC), 1x130 µL (1:100 dilution)
: Sample Diluent Buffer, 1x30 mL
: Antibody Diluent Buffer, 1x10 mL
: ABC Diluent Buffer, 1x10 mL
: TMB Color Developing Agent, 1x10 mL
: TMB Stop Solution, 2N H2SO4, 1x10 mL
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Fibronectin ELISA Kit
High Sensitivity Reactivity: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Fibronectin ELISA KitReactivity: Cow Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Fibronectin ELISA KitReactivity: Human Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Fibronectin ELISA KitReactivity: Mouse Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Fibronectin ELISA KitReactivity: Rat Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum
Fibronectin ELISA KitReactivity: Rat Colorimetric Sandwich ELISA 0.156 μg/mL - 10 μg/mL Plasma, Serum, Tissue Homogenate
Fibronectin ELISA KitReactivity: Pig Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Fibronectin ELISA KitReactivity: Human Colorimetric Competition ELISA 0.781-50 μg/mL Plasma, Serum
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Restrictions
- For Research Use only
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Storage
- 4 °C/-20 °C
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- Fibronectin
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Background
- Fibronectin (FN) also known as LETS, is identified on the surface FN of fibroblasts by labeling with radioactive compounds or specific antibodies. Fibronectin is a 430kD dimeric glycoprotein that exists in 2 forms, termed cellular and plasma fibronectin. Cellular and plasma fibronectins are heterodimers consisting of similar but not identical polypeptides (1). These two forms of FN differ in biologic activity. Fibronectins bind cell surface FNs and various compounds including collagen, fibrin, heparin, DNA, and actin. Because fibronectin stimulates endocytosis in several systems and promotes the clearance of particulate material from the circulation, it could function in the clearance of C1q-coated material such as immune complexes or cellular debris (2). Fibronectins are involved in cell adhesion, cell motility, opsonization, would healing, and maintenance of cell shape. LETS, encoded on chromosome 8, is responsible for the LETS protein expression in humans. Because LETS has been implicated in tumorigenicity and cellular transformation, it is of interest that rearrangement or modifications in the number of chromosome 8 have been associated with certain forms of cancer (3). The standard used in this kit is isolated from human plasma with the molecular mass of 200-250kD.
Target See all Fibronectin ELISA Kits
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