Fibronectin ELISA Kit
Quick Overview for Fibronectin ELISA Kit (ABIN2042467)
Target
See all Fibronectin ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
-
-
Minimum Detection Limit
- 6 pg/mL
-
Cross-Reactivity (Details)
- There is no detectable cross-reactivity with other relevant proteins.
-
Sensitivity
- < 15 pg/mL
-
Components
-
96-well plate pre-coated with anti-Fibronectin, Mouse
Lyophilized recombinant Fibronectin, Mouse standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
-
-
Fibronectin ELISA Kit
High Sensitivity Reactivity: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Fibronectin ELISA KitReactivity: Cow Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Fibronectin ELISA KitReactivity: Human Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Fibronectin ELISA KitReactivity: Mouse Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Fibronectin ELISA KitReactivity: Rat Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum
Fibronectin ELISA KitReactivity: Rat Colorimetric Sandwich ELISA 0.156 μg/mL - 10 μg/mL Plasma, Serum, Tissue Homogenate
Fibronectin ELISA KitReactivity: Pig Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Fibronectin ELISA KitReactivity: Human Colorimetric Competition ELISA 0.781-50 μg/mL Plasma, Serum
-
-
Plate
- Pre-coated
-
Protocol
- Sandwich ELISA quantitative detection kit for Mouse Fibronectin in cell culture supernates, serum and plasma (heparin, EDTA, citrate).Fibronectin (FN) also known as LETS, is identified on the surfFN of fibroblasts by labeling with radioactive compounds or specific antibodies. Fibronectin is a 430,000-dalton dimeric glycoprotein that exists in 2 forms, termed cellular and plasma fibronectin. Cellular and plasma fibronectins are heterodimers consisting of similar but not identical polypeptides.1 These two forms of FN differ in biologic activity. Fibronectins bind cell surfFNs and various compounds including collagen, fibrin, heparin, DNA, and actin. Because fibronectin stimulates endocytosis in several systems and promotes the clearance of particulate material from the circulation, it could function in the clearance of C1q-coated material such as immune complexes or cellular debris.2 Fibronectins are involved in cell adhesion, cell motility, opsonization, would healing, and maintenance of cell shape. LETS, encoded on chromosome 8, is responsible for the LETS protein expression in humans. Because LETS has been implicated in tumorigenicity and cellular transformation, it is of interest that rearrangement or modifications in the number of chromosome 8 have been associated with certain forms of cancer.3
-
Restrictions
- For Research Use only
-
-
-
Storage
- 4 °C/-20 °C
-
-
- Fibronectin
-
Gene ID
- 16412
-
UniProt
- P09055
Target See all Fibronectin ELISA Kits
-
