CX3CL1 ELISA Kit

Catalog No. ABIN2042470

Human CX3CL1 ELISA Kit Colorimetric assay for quantification of Human CX3CL1.

Quick Overview for CX3CL1 ELISA Kit (ABIN2042470)

Target: CX3CL1 (Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 6-10000 pg/mL

Application: ELISA

Product Details CX3CL1 ELISA Kit

Minimum Detection Limit: 6 pg/mL

Cross-Reactivity (Details): There is detectable cross-reactivity with Eotaxin, MCP-1, MCP-2, 6Ckine, MARC < 0.2 % .

Sensitivity: < 50 pg/mL

Components: 96-well plate pre-coated with anti-Fractalkine/CX3CL1, Human
Lyophilized recombinant Fractalkine/CX3CL1, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Application Details

Plate: Pre-coated

Protocol: Sandwich ELISA quantitative detection kit for Human Fractalkine/CX3CL1 in cell culture supernates, serum and plasma (heparin, EDTA), saliva, urine and human milk.Chemokine (C-X3-C motif) ligand 1 (CX3CL1) is a large cytokine protein of 373 amino acids, it contains multiple domains and is the only known member of the CX3C chemokine family. It is also commonly known under the names fractalkine (in humans) and neurotactin (in mice).1, 2 CX3CL1 is produced as a long protein (with 373-amino acid in humans) with an extended mucin-like stalk and a chemokine domain on top. The mucin-like stalk permits it to bind to the surface of certain cells. However a soluble (90kD) version of this chemokine has also been observed. Soluble CX3CL1 potently chemoattracts T cells and monocytes, while the cell-bound chemokine promotes strong adhesion of leukocytes to activated endothelial cells, where it is primarily expressed.2 CX3CL1 elicits its adhesive and migratory functions by interacting with the chemokine receptor CX3CR1.3 Its gene is located on human chromosome 16 along with some CC chemokines known as CCL17 and CCL22.2, 4 It can act as a mediator of smooth muscle cell migration in human atherosclerosis, rather than mediate inflammatory cell recruitment.5

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for CX3CL1

Target: CX3CL1 (Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1))

Alternative Name: Fractalkine/CX3CL1

Gene ID: 6376

UniProt: A0N0N7

Pathways: Synaptic Membrane