HSP27 ELISA Kit
Quick Overview for HSP27 ELISA Kit (ABIN2042492)
Target
See all HSP27 ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
-
-
Minimum Detection Limit
- 62.5 pg/mL
-
Cross-Reactivity (Details)
- There is no detectable cross-reactivity with other relevant proteins.
-
Sensitivity
- < 5 pg/mL
-
Components
-
96-well plate pre-coated with anti-HSP27, Human
Lyophilized recombinant HSP27, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
-
-
Heat Shock Protein 27 (HSP27) ELISA Kit
HSP27 Reactivity: Human Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Heat Shock Protein 27 (HSP27) ELISA KitHSP27 Reactivity: Mouse Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Heat Shock Protein 27 (HSP27) ELISA KitHSP27 Reactivity: Cow Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Heat Shock Protein 27 (HSP27) ELISA KitHSP27 Reactivity: Rabbit Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Heat Shock Protein 27 (HSP27) ELISA KitHSP27 Reactivity: Rat Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Heat Shock Protein 27 (HSP27) ELISA KitHigh Sensitivity HSP27 Reactivity: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
-
-
Plate
- Pre-coated
-
Protocol
- Sandwich ELISA quantitative detection kit for Human HSP27 in cell lysates.Hsp27 is a chaperone of the sHsp (small heat shock protein) group among ubiquitin, -crystallin, Hsp20 and others. The common functions of sHsps are chaperone activity, thermotolerance, inhibition of apoptosis, regulation of cell development, and cell differentiation. They also take part in signal transduction. The HSP27 gene has 3 exons. The mouse Hsp25 gene was mapped to chromosome 5 in a region homologous to 7q in the human. They also mapped the mouse Hsp105 gene to chromosome 5 but suggested that the human homolog is probably on 13q, not chromosome 7. The standard used in this kit is recombinant human HSP27, consisting of 205 amino acids with the molecular mass of 22.7kD.
-
Restrictions
- For Research Use only
-
-
-
Storage
- 4 °C/-20 °C
-
-
- HSP27 (Heat Shock Protein 27 (HSP27))
-
Gene ID
- 3315
-
UniProt
- P04792
-
Pathways
- VEGF Signaling
Target See all HSP27 ELISA Kits
-
