IGF2 ELISA Kit
Quick Overview for IGF2 ELISA Kit (ABIN2042503)
Target
See all IGF2 ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
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Minimum Detection Limit
- 62.5 pg/mL
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Cross-Reactivity (Details)
- There is no detectable cross-reactivity with IGF-1.
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Sensitivity
- < 5 pg/mL
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Components
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96-well plate pre-coated with anti-IGF-2, Rat
Lyophilized recombinant IGF-2, Rat standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
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Plate
- Pre-coated
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Protocol
- Sandwich ELISA quantitative detection kit for Rat IGF-2 in cell culture supernates, serum and plasma (heparin, EDTA).Insulin-like growth factor II is also known as somatomedin A. IGF-2 is a member of the insulin family of polypeptide growth factors that is involved in development and growth.1 It is paternally expressed in the fetus and placenta.2 IGF-II is a mitogen for many cell types and an important modulator of muscle growth and differentiation. IGF-II gene is prevalently expressed during prenatal development and its gene activity is regulated by genomic imprinting, in that the allele inherited from the father is active and the allele inherited from the mother is inactive in most normal tissues.3 IGF-II appears to be induced by placental lactogen during prenatal development.4 It is a mediator of prolactin-induced alveologenesis, prolactin, IGF-2, and cyclin D1, all of which are overexpressed in breast cancers, are components of a developmental pathway in the mammary gland.5
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Restrictions
- For Research Use only
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Storage
- 4 °C/-20 °C
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- IGF2 (Insulin-Like Growth Factor 2 (IGF2))
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Alternative Name
- IGF-2
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Gene ID
- 24483
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UniProt
- P01346
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Pathways
- Hormone Activity, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Carbohydrate Metabolic Process, Activated T Cell Proliferation
Target See all IGF2 ELISA Kits
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