IL-17 ELISA Kit

Catalog No. ABIN2042528

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse IL-17.

Quick Overview for IL-17 ELISA Kit (ABIN2042528)

Target: IL-17 (IL17) (Interleukin 17 (IL17))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6-1000 pg/mL

Application: ELISA

Product Details IL-17 ELISA Kit

Minimum Detection Limit: 15.6 pg/mL

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 1 pg/mL

Components: 96-well plate pre-coated with anti-IL-17, Mouse
Lyophilized recombinant IL-17, Mouse standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Application Details

Plate: Pre-coated

Protocol: Sandwich ELISA quantitative detection kit for Mouse IL-17 in cell culture supernates, serum and plasma (heparin, EDTA).IL-17 is an inflammatory cytokine produced primarily by a unique lineage of CD4 T cells that plays critical roles in the pathogenesis of multiple autoimmune diseases. Interleukin-17 is expressed by activated T cells and is 57% identical to the 17- to 26kD secretory glycoprotein encoded by gene 13 of the herpesvirus saimiri (HVS-13). IL17 induces nuclear factor kappa-B and the expression of IL6, intercellular adhesion molecule-1, granulocyte macrophage colony-stimulating factor,and prostaglandin E2, as well as the maturation of CD34 positive hematopoietic precursors into neutrophils. Anti-IL17 antibodies significantly inhibited osteoclast formation induced by culture media of RA synovial tissues. The standard product used in this kit is recombinant mouse IL-17, consisting of 137 amino acids with the molecular mass of 15.5kD.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for IL-17

Target: IL-17 (IL17) (Interleukin 17 (IL17))

Alternative Name: Interleukin 17 (IL-17)

Gene ID: 16171

UniProt: Q544E6