IL-33 ELISA Kit
Quick Overview for IL-33 ELISA Kit (ABIN2042551)
Target
See all IL-33 (IL33) ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
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Minimum Detection Limit
- 31.2 pg/mL
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Cross-Reactivity (Details)
- There is no detectable cross-reactivity with other relevant proteins.
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Sensitivity
- < 10 pg/mL
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Components
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96-well plate pre-coated with anti-IL-33, Mouse
Lyophilized recombinant IL-33, Mouse standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
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Interleukin 33 (IL33) ELISA Kit
IL33 Reactivity: Human Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 33 (IL33) ELISA KitIL33 Reactivity: Rat Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 33 (IL33) ELISA KitIL33 Reactivity: Mouse Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 33 (IL33) ELISA KitIL33 Reactivity: Human Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Urine
Interleukin 33 (IL33) ELISA KitIL33 Reactivity: Dog Colorimetric Sandwich ELISA 23.44 pg/mL - 1500 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 33 (IL33) ELISA KitIL33 Reactivity: Human Colorimetric Sandwich ELISA 32-4000 pg/mL Cell Lysate, Plasma, Serum
Interleukin 33 (IL33) ELISA KitIL33 Reactivity: Mouse AA 109-266 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Interleukin 33 (IL33) ELISA KitIL33 Reactivity: Mouse Colorimetric Sandwich ELISA 16-1000 pg/mL Cell Lysate, Plasma, Serum
Interleukin 33 (IL33) ELISA KitIL33 Reactivity: Human AA 112-270 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
Interleukin 33 (IL33) ELISA KitIL33 Reactivity: Human Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Lysate, Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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Plate
- Pre-coated
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Protocol
- Sandwich ELISA quantitative detection kit for Mouse IL-33 in cell culture supernates, serum and plasma (heparin, EDTA).Interleukin 33 (IL-33) is a cytokine belonging to the IL-1 superfamily. By genomic sequence analysis, the IL33 gene is mapped to chromosome 9p24.1 and its mouse homolog to a syntenic region on chromosome 19qC1. The induction of type 2 cytokines by IL-33 in vivo is believed to induce the severe pathological changes observed in mucosal organs following administration of IL-33. IL33, an alarmin released from necrotic cells, is necessary for potent CD8 + T cell (CTL) responses to replicating, prototypic RNA, and DNA viruses in mice. IL33 prevented the downregulation of CXCR2 and inhibition of chemotaxis induced by activation of TLR4, and found that IL33 reverses the TLR4-induced reduction of CXCR2 expression via the inhibition of expression of GRK2.
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Restrictions
- For Research Use only
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Storage
- 4 °C/-20 °C
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- IL-33 (IL33) (Interleukin 33 (IL33))
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Alternative Name
- IL-33
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Gene ID
- 77125
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UniProt
- Q8BVZ5
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Pathways
- Production of Molecular Mediator of Immune Response
Target See all IL-33 (IL33) ELISA Kits
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