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Laminin ELISA Kit

This Mouse Laminin ELISA Kit is a Colorimetric ELISA Kit designed to quantify Mouse Laminin.
Catalog No. ABIN2042574

Quick Overview for Laminin ELISA Kit (ABIN2042574)

Target

See all Laminin (LN) ELISA Kits
Laminin (LN)

Reactivity

  • 8
  • 5
  • 4
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Mouse

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

6-10000 pg/mL

Application

ELISA
  • Minimum Detection Limit

    6 pg/mL

    Cross-Reactivity (Details)

    There is no detectable cross-reactivity with other relevant proteins.

    Sensitivity

    < 10 pg/mL

    Components

    96-well plate pre-coated with anti-Laminin, Mouse
    Lyophilized recombinant Laminin, Mouse standard, 10 ng/tube, 2Ea
    Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
    Sample diluent buffer, 30 mL
    Antibody diluent buffer, 10 mL
    ABC diluent buffer, 10 mL
    TMB color developing agent, 10 mL
    TMB stop solution, 10 mL
  • Plate

    Pre-coated

    Protocol

    Sandwich ELISA quantitative detection kit for Mouse Laminin in cell culture supernates, serum and plasma (heparin, EDTA).Laminin is a large basement membrane glycoprotein composed of three subunits designated the A, B1, and B2.1 Laminin has diverse biological functions, which include stimulating epithelial cell growth and differentiation.2 The nucleotide sequence of human laminin A chain has an open reading frame encoding 3075-amino acids.1 The human laminin A chain is at locus 18p11.3.3 The nucleotide sequence of the human laminin B1 reveals a 5358-base pair open reading frame that potentially codes for 1786 amino acids, including 20 amino acids of a presumptive signal peptide.2 The gene for the human laminin-B1 chain has been localized to chromosome 7, band q31.4 The B2 chain consists of six distinct domains, including two domains with alpha-helical, coiled-coil structures, two domains with cysteine-rich homologous repeats, and two globular domains. The amino acid sequences of the B2 and B1 chains demonstrate considerable homology.5

    Restrictions

    For Research Use only
  • Storage

    4 °C/-20 °C
  • Target See all Laminin (LN) ELISA Kits

    Laminin (LN)

    Alternative Name

    Laminin

    Gene ID

    16774

    UniProt

    E9PUR4
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