CCL3 ELISA Kit
Quick Overview for CCL3 ELISA Kit (ABIN2042596)
Target
See all CCL3 ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
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Minimum Detection Limit
- 31.2 pg/mL
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Cross-Reactivity (Details)
- There is no detectable cross-reactivity with other relevant proteins.
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Sensitivity
- < 10 pg/mL
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Components
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96-well plate pre-coated with anti-MIP-1 alpha, Human
Lyophilized recombinant MIP-1 alpha, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
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Plate
- Pre-coated
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Protocol
- Sandwich ELISA quantitative detection kit for Human MIP-1 alpha in cell culture supernates, serum and plasma (heparin, EDTA, citrate).Macrophage inflammatory protein-1 alpha (MIP-1 alpha), also called CCL3, LD78. The cDNA for MIP-1 alpha predicts a mature peptide of 69 amino acids with a molecular mass of 7,889 daltons.1 LD78 is a member of a newly identified superfamily of small inducible proteins involved in inflammatory responses, wound healing and tumorigenesis. MIP-1 alpha is a chemokine that has pro-inflammatory and stem cell inhibitory activities in vitro. It constitutes an important second signal for mast cell degranulation in the conjunctiva in vivo and consequently for acute-phase disease. The standard product used in this kit is recombinant human MIP-1 , consisting of 66 amino acids with the molecular mass of 7.5kD.
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Restrictions
- For Research Use only
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Storage
- 4 °C/-20 °C
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- CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))
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Alternative Name
- MIP-1 alpha
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Gene ID
- 6348
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UniProt
- A0N0R1
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Pathways
- Cellular Response to Molecule of Bacterial Origin, Autophagy
Target See all CCL3 ELISA Kits
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