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CCL3 ELISA Kit

Human CCL3 ELISA Kit Colorimetric assay for quantification of Human CCL3.
Catalog No. ABIN2042596

Quick Overview for CCL3 ELISA Kit (ABIN2042596)

Target

See all CCL3 ELISA Kits
CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))

Reactivity

  • 11
  • 10
  • 10
  • 4
  • 3
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Human

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

31.2-2000 pg/mL

Application

ELISA
  • Minimum Detection Limit

    31.2 pg/mL

    Cross-Reactivity (Details)

    There is no detectable cross-reactivity with other relevant proteins.

    Sensitivity

    < 10 pg/mL

    Components

    96-well plate pre-coated with anti-MIP-1 alpha, Human
    Lyophilized recombinant MIP-1 alpha, Human standard, 10 ng/tube, 2Ea
    Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
    Sample diluent buffer, 30 mL
    Antibody diluent buffer, 10 mL
    ABC diluent buffer, 10 mL
    TMB color developing agent, 10 mL
    TMB stop solution, 10 mL
  • Plate

    Pre-coated

    Protocol

    Sandwich ELISA quantitative detection kit for Human MIP-1 alpha in cell culture supernates, serum and plasma (heparin, EDTA, citrate).Macrophage inflammatory protein-1 alpha (MIP-1 alpha), also called CCL3, LD78. The cDNA for MIP-1 alpha predicts a mature peptide of 69 amino acids with a molecular mass of 7,889 daltons.1 LD78 is a member of a newly identified superfamily of small inducible proteins involved in inflammatory responses, wound healing and tumorigenesis. MIP-1 alpha is a chemokine that has pro-inflammatory and stem cell inhibitory activities in vitro. It constitutes an important second signal for mast cell degranulation in the conjunctiva in vivo and consequently for acute-phase disease. The standard product used in this kit is recombinant human MIP-1 , consisting of 66 amino acids with the molecular mass of 7.5kD.

    Restrictions

    For Research Use only
  • Storage

    4 °C/-20 °C
  • Target See all CCL3 ELISA Kits

    CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))

    Alternative Name

    MIP-1 alpha

    Gene ID

    6348

    UniProt

    A0N0R1

    Pathways

    Cellular Response to Molecule of Bacterial Origin, Autophagy
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