Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

CCL20 ELISA Kit

This Human CCL20 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human CCL20.
Catalog No. ABIN2042598

Quick Overview for CCL20 ELISA Kit (ABIN2042598)

Target

See all CCL20 ELISA Kits
CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

Reactivity

  • 9
  • 8
  • 7
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Human

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

15.6-1000 pg/mL

Application

ELISA
  • Minimum Detection Limit

    15.6 pg/mL

    Cross-Reactivity (Details)

    There is no detectable cross-reactivity with other relevant proteins.

    Sensitivity

    < 1 pg/mL

    Components

    96-well plate pre-coated with anti-MIP-3 alpha/CCL20, Human
    Lyophilized recombinant MIP-3 alpha/CCL20, Human standard, 10 ng/tube, 2Ea
    Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
    Sample diluent buffer, 30 mL
    Antibody diluent buffer, 10 mL
    ABC diluent buffer, 10 mL
    TMB color developing agent, 10 mL
    TMB stop solution, 10 mL
  • Plate

    Pre-coated

    Protocol

    Sandwich ELISA quantitative detection kit for Human MIP-3 alpha in cell culture supernates, serum and plasma (heparin, EDTA).Macrophage Inflammatory Protein 3 (MIP3 ), also called Chemokine, cc motif, ligand 20 (CCL20). The MIP-3alpha/CCL20 gene was cloned and sequenced, revealing a four exon, three intron structure, and was localized by FISH analysis to 2q35-q36. MIP3 is predominantly expressed in lymph nodes, appendix, PBL, fetal liver, fetal lung and several cell lines. MIP3 /CCL20 and its receptor CCR6 are markedly up-regulated in psoriasis, and they may play a role in the recruitment of T cells to lesional psoriatic skin. And Alanine MIP-3 and Serine MIP-3 , the two forms of MIP3 , that differ by one amino acid at the predicted signal peptide cleavage site. Both of them were chemically synthesized and tested for biological activity. And both flu antigen plus IL-2-activated CD4(+) and CD8(+) T lymphoblasts and cord blood-derived dendritic cells responded to Ser and Ala MIP-3 .

    Restrictions

    For Research Use only
  • Storage

    4 °C/-20 °C
  • Target See all CCL20 ELISA Kits

    CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

    Alternative Name

    MIP-3 alpha/ccl20

    Gene ID

    6364

    UniProt

    P78556

    Pathways

    The Global Phosphorylation Landscape of SARS-CoV-2 Infection
You are here:
Chat with us!