CCL20 ELISA Kit
Quick Overview for CCL20 ELISA Kit (ABIN2042598)
Target
See all CCL20 ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
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Minimum Detection Limit
- 15.6 pg/mL
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Cross-Reactivity (Details)
- There is no detectable cross-reactivity with other relevant proteins.
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Sensitivity
- < 1 pg/mL
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Components
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96-well plate pre-coated with anti-MIP-3 alpha/CCL20, Human
Lyophilized recombinant MIP-3 alpha/CCL20, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
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Plate
- Pre-coated
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Protocol
- Sandwich ELISA quantitative detection kit for Human MIP-3 alpha in cell culture supernates, serum and plasma (heparin, EDTA).Macrophage Inflammatory Protein 3 (MIP3 ), also called Chemokine, cc motif, ligand 20 (CCL20). The MIP-3alpha/CCL20 gene was cloned and sequenced, revealing a four exon, three intron structure, and was localized by FISH analysis to 2q35-q36. MIP3 is predominantly expressed in lymph nodes, appendix, PBL, fetal liver, fetal lung and several cell lines. MIP3 /CCL20 and its receptor CCR6 are markedly up-regulated in psoriasis, and they may play a role in the recruitment of T cells to lesional psoriatic skin. And Alanine MIP-3 and Serine MIP-3 , the two forms of MIP3 , that differ by one amino acid at the predicted signal peptide cleavage site. Both of them were chemically synthesized and tested for biological activity. And both flu antigen plus IL-2-activated CD4(+) and CD8(+) T lymphoblasts and cord blood-derived dendritic cells responded to Ser and Ala MIP-3 .
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Restrictions
- For Research Use only
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Storage
- 4 °C/-20 °C
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- CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))
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Alternative Name
- MIP-3 alpha/ccl20
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Gene ID
- 6364
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UniProt
- P78556
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Pathways
- The Global Phosphorylation Landscape of SARS-CoV-2 Infection
Target See all CCL20 ELISA Kits
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