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CCL20 ELISA Kit

Rat CCL20 ELISA Kit Colorimetric assay for quantification of Rat CCL20.
Catalog No. ABIN2042599

Quick Overview for CCL20 ELISA Kit (ABIN2042599)

Target

See all CCL20 ELISA Kits
CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

Reactivity

  • 10
  • 8
  • 6
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Rat

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

7.8-500 pg/mL

Application

ELISA
  • Minimum Detection Limit

    7.8 pg/mL

    Cross-Reactivity (Details)

    There is no detectable cross-reactivity with other relevant proteins.

    Sensitivity

    < 1 pg/mL

    Components

    96-well plate pre-coated with anti-MIP-3 alpha/CCL20, Rat
    Lyophilized recombinant MIP-3 alpha/CCL20, Rat standard, 10 ng/tube, 2Ea
    Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
    Sample diluent buffer, 30 mL
    Antibody diluent buffer, 10 mL
    ABC diluent buffer, 10 mL
    TMB color developing agent, 10 mL
    TMB stop solution, 10 mL
  • Plate

    Pre-coated

    Protocol

    Sandwich ELISA quantitative detection kit for Rat MIP-3 alpha/CCL20 in cell culture supernates, serum and plasma (heparin, EDTA).Macrophage Inflammatory Protein 3 (MIP3 ), also called Chemokine, cc motif, ligand 20 (CCL20). The MIP-3alpha/CCL20 gene was cloned and sequenced, revealing a four exon, three intron structure, and was localized by FISH analysis to 2q35-q36. MIP3 is predominantly expressed in lymph nodes, appendix, PBL, fetal liver, fetal lung and several cell lines. MIP3 /CCL20 and its receptor CCR6 are markedly up-regulated in psoriasis, and they may play a role in the recruitment of T cells to lesional psoriatic skin. And Alanine MIP-3 and Serine MIP-3 , the two forms of MIP3 , that differ by one amino acid at the predicted signal peptide cleavage site. Both of them were chemically synthesized and tested for biological activity. And both flu antigen plus IL-2-activated CD4(+) and CD8(+) T lymphoblasts and cord blood-derived dendritic cells responded to Ser and Ala MIP-3 .

    Restrictions

    For Research Use only
  • Storage

    4 °C/-20 °C
  • Target See all CCL20 ELISA Kits

    CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

    Alternative Name

    MIP-3 alpha/ccl20

    Gene ID

    29538

    UniProt

    P97884

    Pathways

    The Global Phosphorylation Landscape of SARS-CoV-2 Infection
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