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MMP13 ELISA Kit

Human MMP13 ELISA Kit Colorimetric assay for quantification of Human MMP13.
Catalog No. ABIN2042611

Quick Overview for MMP13 ELISA Kit (ABIN2042611)

Target

See all MMP13 ELISA Kits
MMP13 (Matrix Metallopeptidase 13 (Collagenase 3) (MMP13))

Reactivity

  • 12
  • 7
  • 7
  • 5
  • 4
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Human

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

8-5000 pg/mL

Application

ELISA
  • Minimum Detection Limit

    8 pg/mL

    Cross-Reactivity (Details)

    There is no detectable cross-reactivity with other relevant proteins.

    Sensitivity

    < 5 pg/mL

    Components

    96-well plate pre-coated with anti-MMP13, Human
    Lyophilized recombinant MMP13, Human standard, 10 ng/tube, 2Ea
    Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
    Sample diluent buffer, 30 mL
    Antibody diluent buffer, 10 mL
    ABC diluent buffer, 10 mL
    TMB color developing agent, 10 mL
    TMB stop solution, 10 mL
  • Plate

    Pre-coated

    Protocol

    Sandwich ELISA quantitative detection kit for Human MMP13 in cell culture supernates, serum and plasma (heparin).Collagenase 3 is an enzyme that in humans is encoded by the MMP13 gene.[1][2]Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene cleaves type II collagen more efficiently than types I and III. [3] The expression of MMP13 in osteoarthritic cartilage and its activity against type II collagen indicates that the enzyme plays a significant role in cartilage collagen degradation and must, therefore, form part of a complex target for proposed therapeutic interventions based on collagenase inhibition. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3.

    Restrictions

    For Research Use only
  • Storage

    4 °C/-20 °C
  • Target See all MMP13 ELISA Kits

    MMP13 (Matrix Metallopeptidase 13 (Collagenase 3) (MMP13))

    Alternative Name

    MMP13

    Gene ID

    4322

    UniProt

    A8K846
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