MMP13 ELISA Kit
Quick Overview for MMP13 ELISA Kit (ABIN2042611)
Target
See all MMP13 ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
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Minimum Detection Limit
- 8 pg/mL
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Cross-Reactivity (Details)
- There is no detectable cross-reactivity with other relevant proteins.
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Sensitivity
- < 5 pg/mL
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Components
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96-well plate pre-coated with anti-MMP13, Human
Lyophilized recombinant MMP13, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
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SensoLyte® 520 MMP-13 Assay Kit
FRET Fluorometric
SensoLyte® Plus 520 MMP-13 Assay Kit Fluorimetric and Enhanced SelectivityFRET Reactivity: Human Fluorometric
SensoLyte® 490 MMP-13 Assay KitFRET Fluorometric
Matrix Metallopeptidase 13 (Collagenase 3) (MMP13) ELISA KitMMP13 Reactivity: Mouse Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum
Matrix Metallopeptidase 13 (Collagenase 3) (MMP13) ELISA KitMMP13 Reactivity: Rabbit Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 13 (Collagenase 3) (MMP13) ELISA KitMMP13 Reactivity: Human Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 13 (Collagenase 3) (MMP13) ELISA KitMMP13 Reactivity: Rat Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum
Matrix Metallopeptidase 13 (Collagenase 3) (MMP13) ELISA KitMMP13 Reactivity: Rat Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Matrix Metallopeptidase 13 (Collagenase 3) (MMP13) ELISA KitMMP13 Reactivity: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Serum, Tissue Homogenate, Urine
Matrix Metallopeptidase 13 (Collagenase 3) (MMP13) ELISA KitMMP13 Reactivity: Rabbit Colorimetric Sandwich ELISA 93.75 pg/mL - 6000 pg/mL Plasma, Serum, Tissue Homogenate
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Plate
- Pre-coated
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Protocol
- Sandwich ELISA quantitative detection kit for Human MMP13 in cell culture supernates, serum and plasma (heparin).Collagenase 3 is an enzyme that in humans is encoded by the MMP13 gene.[1][2]Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene cleaves type II collagen more efficiently than types I and III. [3] The expression of MMP13 in osteoarthritic cartilage and its activity against type II collagen indicates that the enzyme plays a significant role in cartilage collagen degradation and must, therefore, form part of a complex target for proposed therapeutic interventions based on collagenase inhibition. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3.
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Restrictions
- For Research Use only
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Storage
- 4 °C/-20 °C
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- MMP13 (Matrix Metallopeptidase 13 (Collagenase 3) (MMP13))
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Alternative Name
- MMP13
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Gene ID
- 4322
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UniProt
- A8K846
Target See all MMP13 ELISA Kits
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