sL-Selectin ELISA Kit

Catalog No. ABIN2042664

This Human sL-Selectin ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human sL-Selectin.

Quick Overview for sL-Selectin ELISA Kit (ABIN2042664)

Target: sL-Selectin (Selectin L (Soluble) (sL-Selectin))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 62.5-4000 pg/mL

Application: ELISA

Product Details sL-Selectin ELISA Kit

Minimum Detection Limit: 62.5 pg/mL

Brand: BioAssay™

Analytical Method: Quantitative

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 5 pg/mL

Components: 96-well plate pre-coated with anti-sL-Selectin, Human
Lyophilized recombinant sL-Selectin, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Application Details

Plate: Pre-coated

Protocol: Sandwich ELISA quantitative detection kit for Human L-Selectin in cell culture supernates, serum and plasma (heparin, EDTA, citrate).L-selectin, also known as CD62L, is a cell adhesion molecule found on leukocytes. It belongs to the selectin family of proteins, which recognize sialylated carbohydrate groups. It is cleaved by ADAM17.SELL L-selectin is a cell surface component that is a member of a family of adhesion/homing receptors which play important roles in leukocyte-endothelial cell interactions. The molecule is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4 binding proteins.1L-selectin acts as a "homing receptor" for leukocytes to enter secondary lymphoid tissues via high endothelial venules. Ligands present on endothelial cells will bind to leukocyte expressing L-selectin, slowing leukocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point2.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for sL-Selectin

Target: sL-Selectin (Selectin L (Soluble) (sL-Selectin))

Gene ID: 6402

UniProt: P14151