HSD11B1L ELISA Kit
Human HSD11B1L ELISA Kit, Colorimetric assay for quantification of Human HSD11B1L.
Catalog No. ABIN2042731
$1,214.07
Plus shipping costs $50.00
96 tests
Shipping to:
United States
Delivery in 16 to 21 Business Days
Quick Overview for HSD11B1L ELISA Kit (ABIN2042731)
Target
See all HSD11B1L products
HSD11B1L
(Hydroxysteroid (11-Beta) Dehydrogenase 1-Like (HSD11B1L))
Reactivity
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Detection Range
0.15-10 ng/mL
Application
ELISA
-
-
Minimum Detection Limit
- 0.15 ng/mL
-
Sensitivity
- < 0.065 ng/mL
-
-
-
Plate
- Pre-coated
-
Protocol
- 11-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD11b3) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 11-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD11b3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 11-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD11b3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 11-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD11b3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of 11-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD11b3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
-
Restrictions
- For Research Use only
-
-
-
Storage
- 4 °C/-20 °C
-
-
- HSD11B1L (Hydroxysteroid (11-Beta) Dehydrogenase 1-Like (HSD11B1L))
-
Alternative Name
- 11-beta-Hydroxysteroid Dehydrogenase Type 3 (HSD11b3)
Target See all HSD11B1L products
-
You are here:
