Proteasome 20S ELISA Kit
Quick Overview for Proteasome 20S ELISA Kit (ABIN2042735)
Target
Reactivity
Detection Method
Method Type
Detection Range
Application
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Minimum Detection Limit
- 370.37 ng/mL
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Brand
- BioAssay™
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Sensitivity
- < 135.6 ng/mL
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Plate
- Pre-coated
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Protocol
- 20S-Proteasome (20S-PSM) BioAssay-ELISA Kit (Mouse) employs the competitive inhibition enzyme immunoassay technique. An antibody specific for 20S-Proteasome (20S-PSM) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between Horseradish Peroxidase (HRP) labeled 20S-Proteasome (20S-PSM) and unlabeled 20S-Proteasome (20S-PSM) (Standards or samples) with the pre-coated antibody specific for 20S-Proteasome (20S-PSM). After incubation the unbound conjugate is washed off. The amount of bound HRP conjugate is reverse proportional to the concentration of 20S-Proteasome (20S-PSM) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of 20S-Proteasome (20S-PSM) in the sample.
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Restrictions
- For Research Use only
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Storage
- 4 °C/-20 °C
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- Proteasome 20S
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Alternative Name
- 20S-Proteasome (20S-PSM)
Target
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