ACAT2 ELISA Kit
Quick Overview for ACAT2 ELISA Kit (ABIN2042751)
Target
See all ACAT2 ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
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Minimum Detection Limit
- 0.15 ng/mL
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Sensitivity
- < 0.047 ng/mL
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Acetyl-CoA Acetyltransferase 2 (ACAT2) ELISA Kit
ACAT2 Reactivity: Human Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Acetyl-CoA Acetyltransferase 2 (ACAT2) ELISA KitACAT2 Reactivity: Mouse Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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Plate
- Pre-coated
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Protocol
- Acetyl Coenzyme A Acetyltransferase 2 (ACAT2) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Acetyl Coenzyme A Acetyltransferase 2 (ACAT2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Acetyl Coenzyme A Acetyltransferase 2 (ACAT2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Acetyl Coenzyme A Acetyltransferase 2 (ACAT2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Acetyl Coenzyme A Acetyltransferase 2 (ACAT2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Restrictions
- For Research Use only
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Storage
- 4 °C/-20 °C
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- ACAT2 (Acetyl-CoA Acetyltransferase 2 (ACAT2))
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Alternative Name
- Acetyl Coenzyme A Acetyltransferase 2 (ACAT2)
Target See all ACAT2 ELISA Kits
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