Acetyl-CoA Carboxylase alpha ELISA Kit

Catalog No. ABIN2042755

Rat Acetyl-CoA Carboxylase alpha ELISA Kit Colorimetric assay for quantification of Rat Acetyl-CoA Carboxylase alpha.

Quick Overview for Acetyl-CoA Carboxylase alpha ELISA Kit (ABIN2042755)

Target: Acetyl-CoA Carboxylase alpha (ACACA)

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 3.12-200 ng/mL

Application: ELISA

Product Details Acetyl-CoA Carboxylase alpha ELISA Kit

Minimum Detection Limit: 3.12 ng/mL

Sensitivity: < 1.34 ng/mL

Application Details

Plate: Pre-coated

Protocol: Acetyl Coenzyme A Carboxylase Alpha (ACACa) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Acetyl Coenzyme A Carboxylase Alpha (ACACa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Acetyl Coenzyme A Carboxylase Alpha (ACACa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Acetyl Coenzyme A Carboxylase Alpha (ACACa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Acetyl Coenzyme A Carboxylase Alpha (ACACa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for Acetyl-CoA Carboxylase alpha

Target: Acetyl-CoA Carboxylase alpha (ACACA)

Alternative Name: Acetyl Coenzyme A Carboxylase alpha (ACACa)

Pathways: AMPK Signaling, Ribonucleoside Biosynthetic Process, Warburg Effect