ACTG2 ELISA Kit

Catalog No. ABIN2042761

Human ACTG2 ELISA Kit Colorimetric assay for quantification of Human ACTG2.

Quick Overview for ACTG2 ELISA Kit (ABIN2042761)

Target: ACTG2 (Actin, gamma 2, Smooth Muscle, Enteric (ACTG2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details ACTG2 ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.053 ng/mL

Application Details

Plate: Pre-coated

Protocol: Actin Gamma 2, Smooth Muscle (ACTg2) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Actin Gamma 2, Smooth Muscle (ACTg2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Actin Gamma 2, Smooth Muscle (ACTg2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Actin Gamma 2, Smooth Muscle (ACTg2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Actin Gamma 2, Smooth Muscle (ACTg2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for ACTG2

Target: ACTG2 (Actin, gamma 2, Smooth Muscle, Enteric (ACTG2))

Alternative Name: Actin gamma 2, Smooth Muscle (ACTg2)