alpha Actinin 4 ELISA Kit

Catalog No. ABIN2042765

This Colorimetric ELISA kit is designed for the quantitative measurement of Human alpha Actinin 4.

Quick Overview for alpha Actinin 4 ELISA Kit (ABIN2042765)

Target: alpha Actinin 4 (ACTN4) (Actinin, alpha 4 (ACTN4))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.78-50 ng/mL

Application: ELISA

Product Details alpha Actinin 4 ELISA Kit

Minimum Detection Limit: 0.78 ng/mL

Sensitivity: < 0.24 ng/mL

Application Details

Plate: Pre-coated

Protocol: Actinin Alpha 4 (ACTN4) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Actinin Alpha 4 (ACTN4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Actinin Alpha 4 (ACTN4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Actinin Alpha 4 (ACTN4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Actinin Alpha 4 (ACTN4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for alpha Actinin 4

Target: alpha Actinin 4 (ACTN4) (Actinin, alpha 4 (ACTN4))

Alternative Name: Actinin alpha 4 (ACTN4)

Pathways: Proton Transport