Arc ELISA Kit

Catalog No. ABIN2042773

Rat Arc ELISA Kit Colorimetric assay for quantification of Rat Arc.

Quick Overview for Arc ELISA Kit (ABIN2042773)

Target: Arc (Activity-Regulated Cytoskeleton-Associated Protein (Arc))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details Arc ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.051 ng/mL

Application Details

Plate: Pre-coated

Protocol: Activity Regulated Cytoskeleton Associated Protein (ARC) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Activity Regulated Cytoskeleton Associated Protein (ARC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Activity Regulated Cytoskeleton Associated Protein (ARC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Activity Regulated Cytoskeleton Associated Protein (ARC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Activity Regulated Cytoskeleton Associated Protein (ARC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for Arc

Target: Arc (Activity-Regulated Cytoskeleton-Associated Protein (Arc))

Alternative Name: Activity Regulated Cytoskeleton Associated Protein (ARC)