ADK ELISA Kit

Catalog No. ABIN2042775

The Mouse ADK ELISA Kit (ABIN2042775) is a Colorimetric ELISA Kit designed to quantify Mouse ADK.

Quick Overview for ADK ELISA Kit (ABIN2042775)

Target: ADK (Adenosine Kinase (ADK))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details ADK ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.054 ng/mL

Application Details

Plate: Pre-coated

Protocol: Adenosine Kinase (ADK) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adenosine Kinase (ADK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adenosine Kinase (ADK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adenosine Kinase (ADK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Adenosine Kinase (ADK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for ADK

Target: ADK (Adenosine Kinase (ADK))

Pathways: Ribonucleoside Biosynthetic Process