ADCY10 ELISA Kit

Catalog No. ABIN2042777

This Colorimetric ELISA kit is designed for the quantitative measurement of Rat ADCY10.

Quick Overview for ADCY10 ELISA Kit (ABIN2042777)

Target: ADCY10 (Adenylate Cyclase 10 (Soluble) (ADCY10))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details ADCY10 ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Sensitivity: < 0.125 ng/mL

Application Details

Plate: Pre-coated

Protocol: Adenylate Cyclase 10, Soluble (ADCY10) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adenylate Cyclase 10, Soluble (ADCY10). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adenylate Cyclase 10, Soluble (ADCY10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adenylate Cyclase 10, Soluble (ADCY10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Adenylate Cyclase 10, Soluble (ADCY10) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for ADCY10

Target: ADCY10 (Adenylate Cyclase 10 (Soluble) (ADCY10))

Alternative Name: Adenylate Cyclase 10, Soluble (ADCY10)

Pathways: cAMP Metabolic Process