AMIGO1 ELISA Kit

Catalog No. ABIN2042787

This Human AMIGO1 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human AMIGO1.

Quick Overview for AMIGO1 ELISA Kit (ABIN2042787)

Target: AMIGO1 (Adhesion Molecule with Ig-Like Domain 1 (AMIGO1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details AMIGO1 ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.064 ng/mL

Application Details

Plate: Pre-coated

Protocol: Adhesion Molecule With Ig Like Domain Protein 1 (AMIGO1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adhesion Molecule With Ig Like Domain Protein 1 (AMIGO1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adhesion Molecule With Ig Like Domain Protein 1 (AMIGO1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adhesion Molecule With Ig Like Domain Protein 1 (AMIGO1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Adhesion Molecule With Ig Like Domain Protein 1 (AMIGO1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for AMIGO1

Target: AMIGO1 (Adhesion Molecule with Ig-Like Domain 1 (AMIGO1))

Alternative Name: Adhesion Molecule With Ig Like Domain Protein 1 (AMIGO1)