ADH1 ELISA Kit

Catalog No. ABIN2042801

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse ADH1.

Quick Overview for ADH1 ELISA Kit (ABIN2042801)

Target: ADH1 (Alcohol Dehydrogenase 1 (Class I) (ADH1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 3.12-200 ng/mL

Application: ELISA

Product Details ADH1 ELISA Kit

Minimum Detection Limit: 3.12 ng/mL

Sensitivity: < 1.32 ng/mL

Application Details

Plate: Pre-coated

Protocol: Alcohol Dehydrogenase 1 (ADH1) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alcohol Dehydrogenase 1 (ADH1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alcohol Dehydrogenase 1 (ADH1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alcohol Dehydrogenase 1 (ADH1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Alcohol Dehydrogenase 1 (ADH1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for ADH1

Target: ADH1 (Alcohol Dehydrogenase 1 (Class I) (ADH1))

Alternative Name: Alcohol Dehydrogenase 1 (ADH1)