AOX1 ELISA Kit

Catalog No. ABIN2042803

Cow AOX1 ELISA Kit Colorimetric assay for quantification of Cow AOX1.

Quick Overview for AOX1 ELISA Kit (ABIN2042803)

Target: AOX1 (Aldehyde Oxidase 1 (AOX1))

Reactivity: Cow

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details AOX1 ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Sensitivity: < 0.107 ng/mL

Application Details

Plate: Pre-coated

Protocol: Aldehyde Oxidase 1 (AOX1) BioAssay-ELISA Kit (Bovine) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aldehyde Oxidase 1 (AOX1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aldehyde Oxidase 1 (AOX1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aldehyde Oxidase 1 (AOX1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Aldehyde Oxidase 1 (AOX1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for AOX1

Target: AOX1 (Aldehyde Oxidase 1 (AOX1))