ALDOA ELISA Kit

Catalog No. ABIN2042805

This Human ALDOA ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human ALDOA.

Quick Overview for ALDOA ELISA Kit (ABIN2042805)

Target: ALDOA (Aldolase A, Fructose-Bisphosphate (ALDOA))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5-4000 pg/mL

Application: ELISA

Product Details ALDOA ELISA Kit

Minimum Detection Limit: 62.5 pg/mL

Sensitivity: < 27.2 pg/mL

Application Details

Plate: Pre-coated

Protocol: Aldolase A, Fructose Bisphosphate (ALDOA) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aldolase A, Fructose Bisphosphate (ALDOA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aldolase A, Fructose Bisphosphate (ALDOA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aldolase A, Fructose Bisphosphate (ALDOA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Aldolase A, Fructose Bisphosphate (ALDOA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for ALDOA

Target: ALDOA (Aldolase A, Fructose-Bisphosphate (ALDOA))

Alternative Name: Aldolase A, Fructose Bisphosphate (ALDOA)

Pathways: Ribonucleoside Biosynthetic Process