AZGP1 ELISA Kit

Catalog No. ABIN2042815

Mouse AZGP1 ELISA Kit Colorimetric assay for quantification of Mouse AZGP1.

Quick Overview for AZGP1 ELISA Kit (ABIN2042815)

Target: AZGP1 (alpha-2-Glycoprotein 1, Zinc-Binding (AZGP1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.25-80 ng/mL

Application: ELISA

Product Details AZGP1 ELISA Kit

Minimum Detection Limit: 1.25 ng/mL

Sensitivity: < 0.49 ng/mL

Application Details

Plate: Pre-coated

Protocol: Alpha-2-Glycoprotein 1, Zinc Binding (aZGP1) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-2-Glycoprotein 1, Zinc Binding (aZGP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alpha-2-Glycoprotein 1, Zinc Binding (aZGP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-2-Glycoprotein 1, Zinc Binding (aZGP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Alpha-2-Glycoprotein 1, Zinc Binding (aZGP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for AZGP1

Target: AZGP1 (alpha-2-Glycoprotein 1, Zinc-Binding (AZGP1))

Alternative Name: alpha-2-Glycoprotein 1, Zinc Binding (aZGP1)

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process