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alpha Fetoprotein ELISA Kit

Monkey alpha Fetoprotein ELISA Kit, Colorimetric assay for quantification of Monkey alpha Fetoprotein.
Catalog No. ABIN2042821
$1,205.86
Plus shipping costs $50.00
96 tests
Shipping to: United States
Delivery in 16 to 22 Business Days

Quick Overview for alpha Fetoprotein ELISA Kit (ABIN2042821)

Target

See all alpha Fetoprotein (AFP) ELISA Kits
alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Reactivity

  • 16
  • 8
  • 5
  • 3
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Monkey

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

0.31-20 ng/mL

Application

ELISA
  • Minimum Detection Limit

    0.31 ng/mL

    Sensitivity

    < 0.118 ng/mL
  • Plate

    Pre-coated

    Protocol

    Alpha-Fetoprotein (aFP) BioAssay-ELISA Kit (Monkey) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-Fetoprotein (aFP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alpha-Fetoprotein (aFP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-Fetoprotein (aFP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Alpha-Fetoprotein (aFP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

    Restrictions

    For Research Use only
  • Storage

    4 °C/-20 °C
  • Target See all alpha Fetoprotein (AFP) ELISA Kits

    alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

    Pathways

    C21-Steroid Hormone Metabolic Process
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