AASDHPPT ELISA Kit

Catalog No. ABIN2042831

This Colorimetric ELISA kit is designed for the quantitative measurement of Human AASDHPPT.

Quick Overview for AASDHPPT ELISA Kit (ABIN2042831)

Target: AASDHPPT (Aminoadipate-Semialdehyde Dehydrogenase-phosphopantetheinyl Transferase (AASDHPPT))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details AASDHPPT ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.054 ng/mL

Application Details

Plate: Pre-coated

Protocol: Aminoadipate Semialdehyde Phosphopantetheinyl Transferase (AASDHPPT) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aminoadipate Semialdehyde Phosphopantetheinyl Transferase (AASDHPPT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aminoadipate Semialdehyde Phosphopantetheinyl Transferase (AASDHPPT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aminoadipate Semialdehyde Phosphopantetheinyl Transferase (AASDHPPT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Aminoadipate Semialdehyde Phosphopantetheinyl Transferase (AASDHPPT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for AASDHPPT

Target: AASDHPPT (Aminoadipate-Semialdehyde Dehydrogenase-phosphopantetheinyl Transferase (AASDHPPT))

Alternative Name: Aminoadipate Semialdehyde Phosphopantetheinyl Transferase (AASDHPPT)