Alpha-amylase 1 ELISA Kit

Catalog No. ABIN2042833

Rat Alpha-amylase 1 ELISA Kit Colorimetric assay for quantification of Rat Alpha-amylase 1.

Quick Overview for Alpha-amylase 1 ELISA Kit (ABIN2042833)

Target: Alpha-amylase 1 (AMY1) (Amylase 1, Salivary (AMY1))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.78-50 ng/mL

Application: ELISA

Product Details Alpha-amylase 1 ELISA Kit

Minimum Detection Limit: 0.78 ng/mL

Sensitivity: < 0.28 ng/mL

Application Details

Plate: Pre-coated

Protocol: Amylase Alpha 1, Salivary (AMY1) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Amylase Alpha 1, Salivary (AMY1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Amylase Alpha 1, Salivary (AMY1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Amylase Alpha 1, Salivary (AMY1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Amylase Alpha 1, Salivary (AMY1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for Alpha-amylase 1

Target: Alpha-amylase 1 (AMY1) (Amylase 1, Salivary (AMY1))

Alternative Name: Amylase alpha 1, Salivary (AMY1)