ANGPTL7 ELISA Kit

Catalog No. ABIN2042855

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse ANGPTL7.

Quick Overview for ANGPTL7 ELISA Kit (ABIN2042855)

Target: ANGPTL7 (Angiopoietin-Like 7 (ANGPTL7))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details ANGPTL7 ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Sensitivity: < 0.117 ng/mL

Application Details

Plate: Pre-coated

Protocol: Angiopoietin Like Protein 7 (ANGPTL7) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Angiopoietin Like Protein 7 (ANGPTL7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Angiopoietin Like Protein 7 (ANGPTL7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Angiopoietin Like Protein 7 (ANGPTL7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Angiopoietin Like Protein 7 (ANGPTL7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for ANGPTL7

Target: ANGPTL7 (Angiopoietin-Like 7 (ANGPTL7))

Alternative Name: Angiopoietin Like Protein 7 (ANGPTL7)